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Personal information and Contacts
Born 17/03/1969, Moscow, Russia.
E-mail: alexander.raskind@weizmann.ac.il Web: That page Address: Hanassi Ha-Rishon, 51/7, Rehovot, 76303, Israel |
1986- School 29,advanced study of English language, Moscow, USSR (silver medal).
1994- M.Sc., Chemical engineering with specialization in biotechnology, Moscow Institute of Fine Chemical Technology, Moscow, USSR (Diploma with excellence). Thesis topic: "Cloning, expression and purification of photosystem II D1 protein in Escherichia coli".
2000- Ph.D., Department of Plant Sciences, Weizmann Institute of Science, Rehovot. Thesis topic: "Calcium-dependent protein phosphorylation in the chloroplast thylakoid membrane" (Advisor: Prof. M. Edelman).
1994 Engineer, Shemiakin Institute of Bioorganick Chemistry, Moscow, Russia
1995-99 Ph.D. student, Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
2000- Postdoctoral fellow, Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Sequence analysis and manipulation with GCG (Wisconsin Package), DNA-Strider, MacVector and tools available via internet; PCR primer design using Oligo 5.1; Comparative analysis of protein 2D gels, quantification of densitograms; Structure visualization and analysis using RasMol, iMol, WebLabViewer; Basic statistical techniques (R package); Desktop publishing (up to the press-ready level); Experience using Deneba Canvas, Adobe products (Photoshop, Acrobat, Framemaker), OCR (OmniPage Pro); Design and deployment of dynamic database-driven web sites; Experience with HTML, PHP, MySQL, basic knowledge of GCI; Basic knowledge of UNIX operating system; Mac addict since 1994. Good to expert knowledge of different aspects of Macintosh operating system (especially OSX) and available software.
Molecular biology techniques
Total and messenger RNA isolation, cDNA synthesis; Southern blotting; PCR (including RACE techniques); DNA fragment analysis and oligonucleotide purification by polyacrilamide gel electrophoresis; Cloning, expression and purification of proteins from E. coli; Preparation of transformation-competent E. coli cells.
Protein techniques
Protein separation and isolation by 1 and 2-dimensional electrophoresis; Tricine protein electrophoresis for analysis of short proteins; Silver staining; Thin layer electrophoresis and TLC of peptides; Analysis by partial proteolysis; Affinity chromatography; Separation by ultracentrifugation; Electroelution; Various methods of extraction and precipitation; In vivo radio-isotopic labelling; Fluorography and autoradiography; Preparation of peptides for microsequencing; Analysis of interaction by chemical cross-linking; Immunodetection (western blotting); Immunoprecipitation; In vitro transcription/translation.
Plant-specific techniques
Intact chloroplast isolation and fractionation; Fractionation of chloroplast thylakoid membranes into stroma and grana lamellae; Protein import into isolated intact chloroplasts; Chloroplast protein phosphorylation; Chlorophyll concentration assay; Plant DNA/RNA isolation.
Efimov, V. A., A. F. Fradkov, A. B. Raskind, M. S. Khristin, V. V. Klimov and O. G. Chakhmakhcheva (1994). "Expression of the Barley Psba Gene in Escherichia-Coli Yields a Functional in-Vitro Photosystem-II Protein D1." Febs Letters 348(2): 153-157.
Mattoo, A. K., M. T. Giardi, A. Raskind and M. Edelman (1999). "Dynamic metabolism of photosystem II reaction center proteins and pigments." Physiologia Plantarum 107(4): 454-461.
Kuttner Y.Y., V. Sobolev, A, Raskind and M. Edelman (2003). A consensus binding structure for adenine at the atomic level permits searching for the ligand site in a wide spectrum of adenine-containing complexes Proteins (in press)
Swegle M., A. Raskind, I. Booij-James, M. Edelman and A. Mattoo (2003). Purification, identification, characterization, cloning and expression of a rice calcium-dependent protein kinase that phosphorylates a peptide mimicking the membrane integral D1 protein of Photosystem II. (in preparation)
Raskind A., M. Swegle, V. Kumar, A. Mattoo and M. Edelman. (2003). Cloning and expression of a chloroplast-imported calcium-dependent protein kinase from Spirodela. (in preparation).
1. Prof. Meir Edelman
Plant Sciences Department
Weizmann Institute of Science
Tel. +972-8-9343301
Fax. +072-8-9469124
E-mail: marvin.edelman@weizmann.ac.il
2. Prof. Avihai Danon
Plant Sciences Department
Weizmann Institute of Science
Tel. +972-8-9342382, 2755
Fax. +072-8-9469124
E-mail: avihai.danon@weizmann.ac.il
3. Dr. Vladimir Sobolev
Plant Science Department
Weizmann Institute of Science
Tel. +972-8-9344434
E-mail: vladimir.sobolev@weizmann.ac.il
Last updated 9.03.2003